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rat liver marathon ready premade cdna library  (TaKaRa)


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    Structured Review

    TaKaRa rat liver marathon ready premade cdna library
    Rat Liver Marathon Ready Premade Cdna Library, supplied by TaKaRa, used in various techniques. Bioz Stars score: 92/100, based on 204 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rat+liver+marathon+ready+cdna+library/Rat+Brain+Marathon+-Ready+cDNA/pm25578860-40-11-18
    Average 92 stars, based on 204 article reviews
    rat liver marathon ready premade cdna library - by Bioz Stars, 2026-09
    92/100 stars

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    Related Articles

    Produced:

    Article Title: The fatty acid desaturase 3 gene encodes for different FADS3 protein isoforms in mammalian tissues
    Article Snippet: .. Designed from the Rattus norvegicus chromosome 1 genomic contig NW_047563.2, an oligonucleotide primer (5 ′ -CAG CAA CGA AGA GGA GCA GTG TCC CAG TGG CA-3 ′ ) was produced to screen the rat liver Marathon-ready cDNA library by Rapid Amplifi cation cDNA Ends (RACE) reactions using the advantage-GC 2 PCR kit (Clontech, Saint-Germain-en-Laye, France). ..

    Article Title: The fatty acid desaturase 3 gene encodes for different FADS3 protein isoforms in mammalian tissues
    Article Snippet: .. Designed from the Rattus norvegicus chromosome 1 genomic contig {"type":"entrez-nucleotide","attrs":{"text":"NW_047563.2","term_id":"62642010","term_text":"NW_047563.2"}} NW_047563.2 , an oligonucleotide primer (5′-CAG CAA CGA AGA GGA GCA GTG TCC CAG TGG CA-3′) was produced to screen the rat liver Marathon-ready cDNA library by Rapid Amplification cDNA Ends (RACE) reactions using the advantage-GC 2 PCR kit (Clontech, Saint-Germain-en-Laye, France). ..

    cDNA Library Assay:

    Article Title: The fatty acid desaturase 3 gene encodes for different FADS3 protein isoforms in mammalian tissues
    Article Snippet: .. Designed from the Rattus norvegicus chromosome 1 genomic contig NW_047563.2, an oligonucleotide primer (5 ′ -CAG CAA CGA AGA GGA GCA GTG TCC CAG TGG CA-3 ′ ) was produced to screen the rat liver Marathon-ready cDNA library by Rapid Amplifi cation cDNA Ends (RACE) reactions using the advantage-GC 2 PCR kit (Clontech, Saint-Germain-en-Laye, France). ..

    Article Title: The fatty acid desaturase 3 gene encodes for different FADS3 protein isoforms in mammalian tissues
    Article Snippet: .. Designed from the Rattus norvegicus chromosome 1 genomic contig {"type":"entrez-nucleotide","attrs":{"text":"NW_047563.2","term_id":"62642010","term_text":"NW_047563.2"}} NW_047563.2 , an oligonucleotide primer (5′-CAG CAA CGA AGA GGA GCA GTG TCC CAG TGG CA-3′) was produced to screen the rat liver Marathon-ready cDNA library by Rapid Amplification cDNA Ends (RACE) reactions using the advantage-GC 2 PCR kit (Clontech, Saint-Germain-en-Laye, France). ..

    Article Title: Production of sialylated
    Article Snippet: .. Using these degenerate primers in conjunction with the AP1 primer supplied in a rat liver Marathon-ready cDNA library (Clontech) according to the manufacturer's instructions, a full length ORF was isolated. .. The complete ORF was subsequently ligated into the yeast integration vector pJN347, under the control of the GAPDH promoter using a HIS gene as a positive selection marker.

    Article Title: Production of sialylated
    Article Snippet: .. Using these degenerate primers in conjunction with the AP1 primer supplied in a rat liver Marathon-ready cDNA library (Clontech) according to the manufacturer's instructions, a full length ORF was isolated. ..

    Article Title: Ascorbic acid production from yeast
    Article Snippet: SEQ ID NO: AGD AL049658 2 (Gene no. T17F15.200) AGD homolog Z97060 4 from Brassica ALO U40390, AB009401 6, 8 RGLO J03536 10 ARA Y13134, Z36018 21 (ORF YBR149w) The following program was used for amplification of AGD: 94° C. 5 min 94° C. 45 s 53.5° C. 30 s 72° C. 1 min {close oversize brace} 33 cycles 40 s 72° C. 7 min 4° C. ∞ The following program was used for amplification of ALO: 94° C. 5 min 94° C. 45 s 50° C. 30 s 72° C. 1 min {close oversize brace} 33 cycles 40 s 72° C. 7 min 4° C. ∞ The following program was used for amplification of ARA: 94° C. 5 min 94° C. 45 s 56° C. 30 s 72° C. 1 min {close oversize brace} 33 cycles 40 s 72° C. 7 min 4° C. ∞ The following program was used for amplification of LGDH: 94° C. 5 min 94° C. 45 s 56° C. 30 s 72° C. 1 min {close oversize brace} 33 cycles 40 s 72° C. 7 min 4° C. ∞ The following program was used for amplification of RGLO: 94° C. 30 s 94° C. 5 s {close oversize brace} 33 cycles 72° C. 4 min 72° C. 5 min 4° C. ∞ Template DNA for AGD and LGDH: 50 ng plasmid cDNA library pFL61 Arabidopsis (ATCC #77500 (Minet M. et al, 1992, Plant J., 2, 417-422)). .. Template DNA for RGLO: 0.5 ng rat liver marathon-ready cDNA library (Clontech #7471-1). .. Template DNA for ALO and ARA: 50 ng genomic DNA from S. cerevisiae GRF18U, extracted using a standard method.


    Article Title: Production of l -Ascorbic Acid by Metabolically Engineered Saccharomyces cerevisiae and Zygosaccharomyces bailii
    Article Snippet: .. Template DNA for AGD and LGDH consisted of 50 ng of the plasmid cDNA library pFL61 from A. thaliana ( 16 ) (ATCC 77500); the DNA for Gulo consisted of 0.5 ng of rat liver marathon-ready cDNA library (Clontech 7471-1); for ALO1 and ARA1 , we used 50 ng of genomic DNA from S. cerevisiae GRF18U, extracted by a standard method. ..

    Polymerase Chain Reaction:

    Article Title: The fatty acid desaturase 3 gene encodes for different FADS3 protein isoforms in mammalian tissues
    Article Snippet: .. Designed from the Rattus norvegicus chromosome 1 genomic contig NW_047563.2, an oligonucleotide primer (5 ′ -CAG CAA CGA AGA GGA GCA GTG TCC CAG TGG CA-3 ′ ) was produced to screen the rat liver Marathon-ready cDNA library by Rapid Amplifi cation cDNA Ends (RACE) reactions using the advantage-GC 2 PCR kit (Clontech, Saint-Germain-en-Laye, France). ..

    Article Title: The fatty acid desaturase 3 gene encodes for different FADS3 protein isoforms in mammalian tissues
    Article Snippet: .. Designed from the Rattus norvegicus chromosome 1 genomic contig {"type":"entrez-nucleotide","attrs":{"text":"NW_047563.2","term_id":"62642010","term_text":"NW_047563.2"}} NW_047563.2 , an oligonucleotide primer (5′-CAG CAA CGA AGA GGA GCA GTG TCC CAG TGG CA-3′) was produced to screen the rat liver Marathon-ready cDNA library by Rapid Amplification cDNA Ends (RACE) reactions using the advantage-GC 2 PCR kit (Clontech, Saint-Germain-en-Laye, France). ..

    Amplification:

    Article Title: The fatty acid desaturase 3 gene encodes for different FADS3 protein isoforms in mammalian tissues
    Article Snippet: .. Designed from the Rattus norvegicus chromosome 1 genomic contig {"type":"entrez-nucleotide","attrs":{"text":"NW_047563.2","term_id":"62642010","term_text":"NW_047563.2"}} NW_047563.2 , an oligonucleotide primer (5′-CAG CAA CGA AGA GGA GCA GTG TCC CAG TGG CA-3′) was produced to screen the rat liver Marathon-ready cDNA library by Rapid Amplification cDNA Ends (RACE) reactions using the advantage-GC 2 PCR kit (Clontech, Saint-Germain-en-Laye, France). ..

    Isolation:

    Article Title: Production of sialylated
    Article Snippet: .. Using these degenerate primers in conjunction with the AP1 primer supplied in a rat liver Marathon-ready cDNA library (Clontech) according to the manufacturer's instructions, a full length ORF was isolated. .. The complete ORF was subsequently ligated into the yeast integration vector pJN347, under the control of the GAPDH promoter using a HIS gene as a positive selection marker.

    Article Title: Production of sialylated
    Article Snippet: .. Using these degenerate primers in conjunction with the AP1 primer supplied in a rat liver Marathon-ready cDNA library (Clontech) according to the manufacturer's instructions, a full length ORF was isolated. ..

    Article Title: Production of sialylated
    Article Snippet: .. Using these degenerate primers in conjunction with the AP 1 primer supplied in a rat liver Marathon-ready cDNA library (Clontech) according to the manufacturer's instructions, a full length ORF was isolated. ..

    Plasmid Preparation:

    Article Title: Production of l -Ascorbic Acid by Metabolically Engineered Saccharomyces cerevisiae and Zygosaccharomyces bailii
    Article Snippet: .. Template DNA for AGD and LGDH consisted of 50 ng of the plasmid cDNA library pFL61 from A. thaliana ( 16 ) (ATCC 77500); the DNA for Gulo consisted of 0.5 ng of rat liver marathon-ready cDNA library (Clontech 7471-1); for ALO1 and ARA1 , we used 50 ng of genomic DNA from S. cerevisiae GRF18U, extracted by a standard method. ..



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